VP7: an attachment protein of bluetongue virus for cellular receptors in Culicoides variipennis.
نویسندگان
چکیده
منابع مشابه
VP7: an attachment protein of bluetongue virus for cellular receptors in Culicoides variipennis.
The importance of VP7 of bluetongue virus (BTV) in the binding of BTV to membrane proteins of the BTV vector Culicoides variipennis was investigated. Core BTV particles, prepared from whole viruses, lacked outer proteins VP2 and VP5 and had VP7 exposed. More core particles and whole viruses bound to membrane preparations of adults of C. variipennis and KC cells, which were cultured from this ve...
متن کاملRGD tripeptide of bluetongue virus VP7 protein is responsible for core attachment to Culicoides cells.
Bluetongue virus (BTV) is an arthropod-borne virus transmitted by Culicoides species to vertebrate hosts. The double-capsid virion is infectious for Culicoides vector and mammalian cells, while the inner core is infectious for only Culicoides-derived cells. The recently determined crystal structure of the BTV core has revealed an accessible RGD motif between amino acids 168 to 170 of the outer ...
متن کاملDetection of bluetongue virus antigens in Culicoides variipennis by enzyme immunoassay.
A solid-phase enzyme immunoassay (EIA) was developed to detect bluetongue (BT) virus antigens in infected cell cultures and in suspensions of infected Culicoides variipennis midges. The technique was equally sensitive for detecting the five U.S. BT virus serotypes (2, 10, 11, 13, and 17) in cell cultures. EIA reliably detected about 3.8 log10 median tissue culture infective doses per ml of BT v...
متن کاملMixed infection of Culicoides variipennis with bluetongue virus serotypes 10 and 17: evidence for high frequency reassortment in the vector.
The primary vector species for bluetongue virus (BTV) in the United States, Culicoides variipennis, was orally infected with BTV serotype 10, BTV serotype 17, or a mixture of the two viruses. The recovery of virus from the infected flies was low following a period of extrinsic incubation. Electrophoretic analysis of progeny virus from singly infected flies revealed that only the parental electr...
متن کاملDetection of bluetongue virus serotype 17 in Culicoides variipennis by nucleic acid blot and sandwich hybridization techniques.
Molecular hybridization techniques were developed for the detection and surveillance of bluetongue virus (BTV) serotype 17 in the insect vector Culicoides variipennis, a biting midge. Radiolabeled RNA and cDNA probes were generated from sequences of the L3 segment of BTV serotype 17. These probes were used to detect BTV RNA in pools of infected C. variipennis by hybridizing the probes directly ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1997
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-78-7-1617